Conventional PCR systems require users to set a predetermined number of cycles based on assay- and sample-specific factors. When performing single-cell sequencing experiments, this requires knowledge ...

In RNA-seq library preparation, selecting the appropriate number of PCR cycles is a critical balancing act to avoid overcycling.

In the rapidly advancing life science fields, endpoint polymerase chain reaction (PCR), real-time PCR (qPCR), and digital PCR (dPCR) have become indispensa | Ebooks ...

Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA and RNA. PCR involves the enzymatic amplification of ...

PCR is a technique used to amplify target DNA in a sample. It’s a well-known method that has undergone numerous modifications to enhance its capabilities. This year, it’s turning 40 years old. PCR has ...

Limited sample material and insufficient DNA input pose significant challenges for downstream analysis in various laboratory settings. To overcome this issue, isothermal amplification techniques have ...

The development of the polymerase chain reaction (PCR) in 1983 by Kary Mullis and coworkers revolutionized molecular biology, allowing scientists to amplify DNA sequences for various applications. 1 ...

PCR enables precise copying of DNA methylation during DNA amplification, overcoming a fundamental limitation of PCR, and unlocks new opportunities for sensitive detection of cancer, chronic disease, ...

In vitro diagnostics (IVD) is an umbrella term for tests conducted on blood or tissue samples to detect diseases, determine the efficacy of novel or established treatments, and monitor health.