Conventional PCR systems require users to set a predetermined number of cycles based on assay- and sample-specific factors. When performing single-cell sequencing experiments, this requires knowledge ...

When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

In the rapidly advancing life science fields, endpoint polymerase chain reaction (PCR), real-time PCR (qPCR), and digital PCR (dPCR) have become indispensa | Ebooks ...

Failed polymerase chain reactions (PCRs) are a common cause of frustration in the lab. While a typical PCR protocol is quite straightforward, scientists must optimize each step to amplify the intended ...

Gene-Analysis Studies Advanced by Use of Low-Volume High-Throughput Real-Time PCR This article describes three gene-analysis studies using the Roche LightCycler® 1536 Real-Time PCR System (Figure 1).

Programmable RNA base editors are excellent tools to modify RNA transcripts at precise locations or designated hotspots. Post editing, it is critical to provide precise and accurate quantification for ...

Medically reviewed by Jordana Haber Hazan, MD Key Takeaways PCR tests are very accurate and can find the virus even in people without symptoms.Rapid tests can be done at home and show results in ...