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cDNA Synthesis By Reverse Transcription

YouTubeLucas Learns

cDNA Synthesis By Reverse Transcription

Normally DNA is transcribed into mRNA but this process can also be reversed. To do this we can utilize the 3’-Poly A tail which all mRNA have in common. Since this tail only contains adenosine nucleotides, we can use it as a location for our primer. We can use an Oligo (dt) Primer which contains a sequence of 12-20 thymine nucleotides ...

30.7K viewsDec 7, 2022

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